Quarterly Journal of Science and Practice
M. V. Shmidt, A. V. Smirnov, N. Ttyurenkov, D. A. Bakulin, Y. I. Velikorodnaya, V. V. Kolomycev , L. V. Goryunova
Волгоградский государственный медицинский университет, Волгоград, Россия; Волгоградский медицинский научный центр, Волгоград, Россия
Purpose of the study. Analysis of the correlation between the nature and degree of pathomorphological changes and the ex-pression of BDNF in various parts of the brain during the experimental reproduction of streptozotocin-induced diabetes mellitus. The experimental study was carried out on 30 white outbred laboratory rats aged 12 months. Modeling of streptozotocin-induced diabetes mellitus lasted 6 months. Sections 5-7 pm thick were stained with thionin using the Nissl method. Brain-derived neurotrophic factor (BDNF) was detected by immunohistochemistry using primary antibodies to the BDNF protein. Statistical data processing was carried out using the Statistica 6.0 software package (StatSoft, USA). Differences between groups were assessed using the Mann - Whitney U-test and were considered statistically significant at p < 0.05. It was revealed that morphological changes in the brain during experi¬mental modeling of diabetic encephalopathy are characterized by a decrease in the cytoplasmic expression of the BDNF protein in the motor, somatosensory, auditory areas of the cerebral cortex and in the CA1, CA3 fields of the hippocampus in neurons with signs of re¬versible changes, as well as in neurons without significant pathomorphological signs of damage, which apparently contributes to a de¬crease in the resistance of neocortex and archicortex neurons to damage in diabetes mellitus.
brain, diabetes mellitus, diabetic encephalopathy
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